MECHANISTIC DEDUCTIONS FROM ISOTOPE EFFECTS IN MULTIREACTANT ENZYME MECHANISMS

被引：173
作者：
COOK, PF [1 ]
CLELAND, WW [1 ]
机构：
[1] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706
来源：
BIOCHEMISTRY | 1981年 / 20卷 / 07期
关键词：
D O I：
10.1021/bi00510a013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：1790 / 1796
页数：7
相关论文
共 18 条
[1]  
AINSLIE GR, 1972, J BIOL CHEM, V247, P946
[2]   USE OF ISOTOPE EFFECTS TO DEDUCE THE CHEMICAL MECHANISM OF FUMARASE [J].
BLANCHARD, JS ;
CLELAND, WW .
BIOCHEMISTRY, 1980, 19 (19) :4506-4513
[3]   USES AND LIMITATIONS OF MEASUREMENTS OF RATES OF ISOTOPIC EXCHANGE AND INCORPORATION IN CATALYZED REACTIONS [J].
BOYER, PD .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (02) :387-410
[4]  
Cleland W W, 1979, Methods Enzymol, V63, P103
[5]  
Cleland W W, 1980, Methods Enzymol, V64, P104
[6]   PARTITION ANALYSIS AND CONCEPT OF NET RATE CONSTANTS AS TOOLS IN ENZYME-KINETICS [J].
CLELAND, WW .
BIOCHEMISTRY, 1975, 14 (14) :3220-3224
[7]  
CLELAND WW, 1977, ISOTOPE EFFECTS ENZY, P153
[8]   PH VARIATION OF ISOTOPE EFFECTS IN ENZYME-CATALYZED REACTIONS .2. ISOTOPE-DEPENDENT STEP NOT PH DEPENDENT - KINETIC MECHANISM OF ALCOHOL-DEHYDROGENASE [J].
COOK, PF ;
CLELAND, WW .
BIOCHEMISTRY, 1981, 20 (07) :1805-1816
[9]   PH VARIATION OF ISOTOPE EFFECTS IN ENZYME-CATALYZED REACTIONS .1. ISOTOPE-DEPENDENT AND PH-DEPENDENT STEPS THE SAME [J].
COOK, PF ;
CLELAND, WW .
BIOCHEMISTRY, 1981, 20 (07) :1797-1805
[10]   PRIMARY AND SECONDARY DEUTERIUM-ISOTOPE EFFECTS ON EQUILIBRIUM-CONSTANTS FOR ENZYME-CATALYZED REACTIONS [J].
COOK, PF ;
BLANCHARD, JS ;
CLELAND, WW .
BIOCHEMISTRY, 1980, 19 (21) :4853-4858
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