ROLES OF ARG(231) AND TYR(284) OF THERMUS-THERMOPHILUS ISOCITRATE DEHYDROGENASE IN THE COENZYME SPECIFICITY

被引：8
作者：
YAOI, T
MIYAZAKI, K
OSHIMA, T
机构：
[1] Department of Life Science, Tokyo Institute of Technology, Yokohama, 227, Nagatsuta
来源：
FEBS LETTERS | 1994年 / 355卷 / 02期
关键词：
THERMOPHILE ISOCITRATE DEHYDROGENASE; SITE-DIRECTED MUTAGENESIS; COENZYME SPECIFICITY; THERMUS THERMOPHILUS;
D O I：
10.1016/0014-5793(94)01191-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The coenzyme binding site of isocitrate dehydrogenase from Thermus thermophilus was analyzed by site-directed mutagenesis. The mutation analysis revealed that Arg(231) and Tyr(284) are involved in the discrimination between NAD and NADP, suggesting that these two residues interact with 2'-phosphate group of NADP.
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收藏
页码：171 / 172
页数：2
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