IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF MICROSOMAL ALANINE AMINOPEPTIDASE

被引：5

作者：

KETTMANN, U

HUMBEL, B

HOLZHAUSEN, HJ

BAHN, H

AURICH, H

机构：

[1] UNIV UTRECHT, DEPT CELL BIOL, UTRECHT, NETHERLANDS

[2] MARTIN LUTHER UNIV, INST PATHOL ANAT, O-4010 HALLE, GERMANY

来源：

ACTA HISTOCHEMICA | 1992年 / 93卷 / 01期

关键词：

D O I：

10.1016/S0065-1281(11)80233-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.

引用

页码：333 / 340

页数：8

共 19 条

[1] RESIN DEVELOPMENT FOR ELECTRON-MICROSCOPY AND AN ANALYSIS OF EMBEDDING AT LOW-TEMPERATURE
CARLEMALM, E
GARAVITO, RM
VILLIGER, W
[J]. JOURNAL OF MICROSCOPY-OXFORD, 1982, 126 (MAY): : 123 - 143
[2] DANIELSEN EM, 1987, MAMMALIAN ECTOENZYME, P47
[3] ON THE PREPARATION OF CRYOSECTIONS FOR IMMUNOCYTOCHEMISTRY
GRIFFITHS, G
MCDOWALL, A
BACK, R
DUBOCHET, J
[J]. JOURNAL OF ULTRASTRUCTURE RESEARCH, 1984, 89 (01): : 65 - 78
[4] HANSEN GH, 1987, EUR J CELL BIOL, V43, P253
[5] Humbel B., 1985, P175
[6] RECONSTITUTION OF PURIFIED DIPEPTIDYL PEPTIDASE .4. A COMPARISON WITH AMINOPEPTIDASE N WITH RESPECT TO MORPHOLOGY AND INFLUENCE OF ANCHORING PEPTIDE ON FUNCTION
HUSSAIN, MM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 815 (02) : 306 - 312
[7] HUTTER HJ, 1986, BIOMED BIOCHIM ACTA, V45, P845
[8] HUTTER HJ, 1972, WISS BEITR HALLE WIT, P10
[9] TOPOLOGY OF MICROVILLAR MEMBRANE HYDROLASES OF KIDNEY AND INTESTINE
KENNY, AJ
MAROUX, S
[J]. PHYSIOLOGICAL REVIEWS, 1982, 62 (01) : 91 - 128
[10] Kenny AJ, 1987, MAMMALIAN ECTOENZYME, P169

← 1 2 →