PRODUCTION OF PETITES BY CELL-CYCLE MUTANTS OF SACCHAROMYCES-CEREVISIAE DEFECTIVE IN DNA-SYNTHESIS

被引:24
作者
NEWLON, CS
LUDESCHER, RD
WALTER, SK
机构
[1] Department of Zoology, University of Iowa, Iowa City, 52242, IA
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 169卷 / 02期
关键词
D O I
10.1007/BF00271670
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mutations in two genes (cdc8 and cdc21) required for nuclear and mitochondrial DNA synthesis in Saccharomyces cerevisiae result in a 6- to 11-fold increase in the rate of mitotic segregation of petites at the permissive temperature. The defect in DNA replication and the increased rate of petite production result from the same mutation since the two phenotypes cosegregate and corevert. Most of the petites isolated from strains carrying mutations in cdc8 and cdc21 contain mtDNA. Therefore, the petites do not result simply from an underreplication of mitochondrial DNA. The mutation rates for nuclear and mitochondrial genes are the same in cdc8, cdc21 and their wild-type parent. Therefore the petites are unlikely to result from an increase in the rate of base pair substitution. © 1979 Springer-Verlag.
引用
收藏
页码:189 / 194
页数:6
相关论文
共 20 条