CLONING AND SEQUENCING OF BETA-MANNANASE GENE FROM BACILLUS-SUBTILIS NM-39

被引：28
作者：
MENDOZA, NS
ARAI, M
SUGIMOTO, K
UEDA, M
KAWAGUCHI, T
JOSON, LM
机构：
[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT APPL BIOL CHEM, SAKAI, OSAKA 591, JAPAN
[2] IND TECHNOL DEV INST, DEPT SCI & TECHNOL, MANILA, PHILIPPINES
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1243卷 / 03期
关键词：
BACTILLUS SUBTILIS; MANNANASE; CLONING; NUCLEOTIDE SEQUENCE;
D O I：
10.1016/0304-4165(95)00011-Y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene encoding beta-mannanase from Bacillus subtilis NM-39 was cloned into Escherichia coli DH5 alpha by using pUC 18 and its nucleotide sequence was determined. The beta-mannanase gene was 1080 base pairs long and encoded a mature protein of 336 amino acids and a signal peptide of 24 amino acids. The deduced amino acid sequence of the cloned mannanase showed sequence homology with mannanase from alkalophilic Bacillus sp. strain AM-001 (about 50%).
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收藏
页码：552 / 554
页数：3
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