THE MAPPING OF TRANSGENES BY FLUORESCENCE IN-SITU HYBRIDIZATION ON G-BANDED MOUSE CHROMOSOMES

被引：26

作者：

SHI, YP ^{[1
]}

HUANG, TT ^{[1
]}

CARLSON, EJ ^{[1
]}

EPSTEIN, CJ ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,BOX 0748,SAN FRANCISCO,CA 94143

来源：

MAMMALIAN GENOME | 1994年 / 5卷 / 06期

关键词：

D O I：

10.1007/BF00356551

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive method for the mapping of transgenes and other genes in the mouse genome is described. This technique combines high-resolution G-banding and fluorescence in situ hybridization (FISH) with either biotin/avidin-FITC or digoxigenin-anti-digoxigenin-FITC, the latter being the more sensitive. Banding patterns are obtained with trypsin/Giemsa-treated slides, and sensitivity is greatly increased by the use of mouse Cot-1 DNA. With this protocol, four different 14.5-kb human Cu/Zn-superoxide dismutase transgene insertions ranging in copy number from 2 to 8 have been localized to four different mouse chromosomes. The utility and sensitivity of this procedure were verified with a Chromosome (Chr) 16-specific cosmid probe, H22, as well as with the mapping of a high-copy-number human beta-amyloid/A4 transgene.

引用

页码：337 / 341

页数：5

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