CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF BOVINE LIVER MITOCHONDRIAL ALDEHYDE DEHYDROGENASE

被引：9
作者：
HURLEY, TD
WEINER, H
机构：
[1] INDIANA UNIV, SCH MED, DEPT MOLEC BIOL, INDIANAPOLIS, IN 46202 USA
[2] PURDUE UNIV, DEPT BIOCHEM, W LAFAYETTE, IN 47907 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 04期
关键词：
ALDEHYDE DEHYDROGENASE; CRYSTALLIZATION; DIFFRACTION;
D O I：
10.1016/0022-2836(92)90536-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aldehyde dehydrogenase from bovine liver mitochondria has been crystallized using the sitting drop method of vapor diffusion at 22 °C. The crystals formed from solutions containing, 40 mm-sodium citrate, 1 mm-NAD+ and 21% to 24% polyethylene glycol 3400 (pH 5·3 to 5·5). X-ray diffraction data collected from these crystals indicate that the crystals belong to the orthorhombic space group P212121 with cell dimensions of a = 153·7 A ̊, b = 159·37 A ̊ and c = 101·45 A ̊. The crystals diffract to at least 2·9 Å and a tetramer may comprise the asymmetric unit. © 1992.
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页码：1255 / 1257
页数：3
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