3-DIMENSIONAL READOUT OF FLASH X-RAY IMAGES OF LIVING SPERM IN WATER BY ATOMIC-FORCE MICROSCOPY

被引：80

作者：

TOMIE, T ^{[1
]}

SHIMIZU, H ^{[1
]}

MAJIMA, T ^{[1
]}

YAMADA, M ^{[1
]}

KANAYAMA, T ^{[1
]}

KONDO, H ^{[1
]}

YANO, M ^{[1
]}

ONO, M ^{[1
]}

机构：

[1] NIKON INC,TSUKUBA LAB,TSUKUBA,IBARAKI 30026,JAPAN

来源：

SCIENCE | 1991年 / 252卷 / 5006期

关键词：

D O I：

10.1126/science.2024121

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subpicosecond x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.

引用

页码：691 / 693

页数：3

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