MUSCLE GROWTH IN RESPONSE TO MECHANICAL STIMULI

被引:155
作者
GOLDSPINK, DF [1 ]
COX, VM [1 ]
SMITH, SK [1 ]
EAVES, LA [1 ]
OSBALDESTON, NJ [1 ]
LEE, DM [1 ]
MANTLE, D [1 ]
机构
[1] NEWCASTLE GEN HOSP, REG NEUROL CTR, DEPT NEUROCHEM, NEWCASTLE UPON TYNE NE4 6BE, TYNE & WEAR, ENGLAND
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1995年 / 268卷 / 02期
关键词
STRETCH COMBINED WITH ELECTRICAL STIMULATION; PROTEIN SYNTHESIS; PROTEOLYSIS; RIBONUCLEIC ACID; DEOXYRIBONUCLEIC ACID; INSULIN-LIKE GROWTH FACTOR; C-FOS; C-JUN;
D O I
10.1152/ajpendo.1995.268.2.E288
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The relative merits of the separate and combined uses of stretch and electrical stimulation at 10 Hz in influencing the rates of protein synthesis in vivo, proteolysis, and the growth of the extensor digitorum longus muscle have been investigated after 3 days in the rabbit. Continuous electrical stimulation failed to change muscle protein turnover or growth. Static stretch caused significant adaptive growth, with increases in c-fos, c-jun, and insulin-like growth factor I (IGF-I; 12-fold) mRNA levels, and protein (19%), RNA (128%), and DNA (45%) contents. Both the fractional (138%) and total (191%) rates of protein synthesis increased with stretch, correlating with increased ribosomal capacities. Combining stretch and electrical stimulation increased the mRNA concentration of IGF-I (40-fold). The adaptive growth was greater (35%), with massive increases in the nucleic acids (185 and 300%), ribosomal capacities (230%), and the rates of protein synthesis (345 and 450%). Large increases (i.e., 200-400%) in cathepsins B and L and dipeptidyl aminopeptidase I activities during stretch, with or without stimulation, suggest a role for these enzymes in tissue remodeling during muscle hypertrophy.
引用
收藏
页码:E288 / E297
页数:10
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