ISOLATION OF CDNA CLONE ENCODING HUMAN HOMOLOG OF SENESCENCE MARKER PROTEIN-30 (SMP30) AND ITS LOCATION ON THE X-CHROMOSOME

被引：55
作者：
FUJITA, T
MANDEL, JL
SHIRASAWA, T
HINO, O
SHIRAI, T
MARUYAMA, N
机构：
[1] TOKYO METROPOLITAN INST GERONTOL,DEPT MOLEC PATHOL,ITABASHI KU,TOKYO 173,JAPAN
[2] FAC MED STRASBOURG,INSERM,U184,STRASBOURG,FRANCE
[3] FAC MED STRASBOURG,CNRS,GENET MOLEC EUCARYOTES LAB,F-67085 STRASBOURG,FRANCE
[4] CHRU,STRASBOURG,FRANCE
[5] JAPANESE FDN CANC RES,INST CANC,DEPT EXPTL PATHOL,TOKYO 170,JAPAN
[6] JUNTENDO UNIV,SCH MED,DEPT PATHOL,TOKYO 113,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1263卷 / 03期
关键词：
AGING; CALCIUM BINDING PROTEIN; CDNA; SENESCENCE MARKER PROTEIN-30 (SMP30); X CHROMOSOME; (HUMAN); (LIVER);
D O I：
10.1016/0167-4781(95)00120-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated and characterized a cDNA clone encoding human homologue of senescence marker protein-30 (SMP30), a calcium binding protein also called regucalcin (RC). This clone (pHSMP6) has 1356 base pairs (bp) and contains an open reading frame of 897 bp, which encodes 299 amino acids. The estimated molecular weight of the deduced polypeptide is 33 250 and pI is 5.836. The homology of amino acid sequences between human homologue and rat SMP30 is 88.6%. Using pHSMP6 as a probe, the chromosomal location of the human homologue of SMP30 gene was determined, The results of regional mapping using a panel of 11 rodent-human somatic hybrids indicated that the gene is located in the p11.3-q11.2 segment of the X chromosome. This gene thus could be a candidate for one of the X-linked diseases mapped to this regions,
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页码：249 / 252
页数：4
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