SOLUTION BEHAVIOR OF ALPHA-CHYMOTRYPSIN DISSOLVED IN NONPOLAR ORGANIC-SOLVENTS VIA HYDROPHOBIC ION-PAIRING

被引：29

作者：

MEYER, JD ^{[1
]}

MATSUURA, JE ^{[1
]}

KENDRICK, BS ^{[1
]}

EVANS, ES ^{[1
]}

EVANS, GJ ^{[1
]}

MANNING, MC ^{[1
]}

机构：

[1] UNIV COLORADO,HLTH SCI CTR,SCH PHARM,DEPT PHARMACEUT SCI,DENVER,CO 80262

来源：

BIOPOLYMERS | 1995年 / 35卷 / 05期

关键词：

D O I：

10.1002/bip.360350504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dissolution of alpha-chymotrypsin in nonpolar organic solvents can be achieved using hydrophobic ion pairing, whereby the polar counterions ave replaced by a stoichiometric number of detergent molecules. Using Aerosol OT [AOT, sodium bis(2-octyl)sulfosuccinate], it is possible to partition significant amounts of the enzyme into alkanes and chlorocarbons. Apparent solubility in isooctane is greater than 1 mg/mL (80 mu M). Necessary conditions for achieving effective partitioning of alpha-chymotrypsin into these solvents are described. Using CD spectroscopy, it can be shown that the AOT-alpha-chymotrypsin (CMT) complex retains its native secondary and tertiary structure when dissolved in alkanes, and that the globular structure is stable to more than 100 degrees C. In contrast, alpha-chymotrypsin unfolds at 54 degrees C in aqueous solution. The relative solubility of the AOT-CMT complex in a variety of alkanes and chloracarbons is also reported. The native structure of alpha-chymotrypsin is maintained in carbon tetrachloride, but not in methylene chloride or chloroform. (C) 1995 John Wiley & Sons, Inc.

引用

页码：451 / 456

页数：6

共 12 条

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