DIRECT EVIDENCE FOR PROTEIN-KINASE-C INVOLVEMENT IN INSULIN-STIMULATED HEXOSE UPTAKE

被引：15
作者：
COOPER, DR
WATSON, JE
HERNANDEZ, H
YU, BZ
STANDAERT, ML
WAYS, DK
ARNOLD, TT
ISHIZUKA, T
FARESE, RV
机构：
[1] UNIV S FLORIDA, COLL MED, DEPT MED, TAMPA, FL 33612 USA
[2] E CAROLINA UNIV, SCH MED, DEPT MED, GREENVILLE, NC 27834 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 01期
关键词：
D O I：
10.1016/0006-291X(92)92361-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Insulin has been reported to translocate protein kinase C (PKC) in rat adipocytes, and activation of PKC by phorbol esters is known to increase hexose uptake in these cells (1,2). To test the hypothesis that PKC may participate in insulin-stimulated hexose uptake, adipocytes were partially depleted of protein kinase C by overnight phorbol ester treatment, thereby impairing insulin effects on hexose uptake. Purified PKC was then introduced into these PKC-depleted adipocytes by electropermeabilization, and this fully restored insulin-stimulated hexose uptake. These findings provide direct evidence that PKC is required for insulin- stimulated hexose uptake. © 1992.
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收藏
页码：142 / 148
页数：7
相关论文
共 26 条
[1]   INSULIN STIMULATION OF GLUCOSE-METABOLISM IN RAT ADIPOCYTES - POSSIBLE IMPLICATION OF PROTEIN-KINASE-C [J].
CHERQUI, G ;
CARON, M ;
WICEK, D ;
LASCOLS, O ;
CAPEAU, J ;
PICARD, J .
ENDOCRINOLOGY, 1986, 118 (05) :1759-1769
[2]   DECREASED INSULIN RESPONSIVENESS IN FAT-CELLS RENDERED PROTEIN-KINASE C-DEFICIENT BY A TREATMENT WITH A PHORBOL ESTER [J].
CHERQUI, G ;
CARON, M ;
WICEK, D ;
LASCOLS, O ;
CAPEAU, J ;
PICARD, J .
ENDOCRINOLOGY, 1987, 120 (05) :2192-2194
[3]   LONG-TERM TREATMENT OF ISOLATED RAT SOLEUS MUSCLE WITH PHORBOL ESTER LEADS TO LOSS OF CONTRACTION-INDUCED GLUCOSE-TRANSPORT [J].
CLELAND, PJF ;
ABEL, KC ;
RATTIGAN, S ;
CLARK, MG .
BIOCHEMICAL JOURNAL, 1990, 267 (03) :659-663
[4]   RETENTION OF SPECIFIC PROTEIN KINASE-C ISOZYMES FOLLOWING CHRONIC PHORBOL ESTER TREATMENT IN BC3H-1 MYOCYTES [J].
COOPER, DR ;
WATSON, JE ;
ACEVEDODUNCAN, M ;
POLLET, RJ ;
STANDAERT, ML ;
FARESE, RV .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (01) :327-334
[5]  
COOPER DR, 1987, J BIOL CHEM, V262, P3633
[6]   INSULIN AND GLUCOSE MODULATE PROTEIN KINASE-C ACTIVITY IN RAT ADIPOCYTES [J].
DRAZNIN, B ;
LEITNER, JW ;
SUSSMAN, KE ;
SHERMAN, NA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (01) :570-575
[7]   INSULIN, OXYTOCIN, AND VASOPRESSIN STIMULATE PROTEIN KINASE-C ACTIVITY IN ADIPOCYTE PLASMA-MEMBRANES [J].
EGAN, JJ ;
SALTIS, J ;
WEK, SA ;
SIMPSON, IA ;
LONDOS, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1052-1056
[8]   INSULIN RAPIDLY INCREASES DIACYLGLYCEROL BY ACTIVATING DENOVO PHOSPHATIDIC-ACID SYNTHESIS [J].
FARESE, RV ;
KONDA, TS ;
DAVIS, JS ;
STANDAERT, ML ;
POLLET, RJ ;
COOPER, DR .
SCIENCE, 1987, 236 (4801) :586-589
[9]  
FARESE RV, 1992, IN PRESS BIOCH J
[10]  
GARVEY WT, 1987, J BIOL CHEM, V262, P189
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