IDENTIFICATION, CHARACTERIZATION AND STEREOTAXIC MAPPING OF INTRA-NEURONAL ALPHA-MELANOCYTE STIMULATING HORMONE-LIKE IMMUNOREACTIVE PEPTIDES IN DISCRETE REGIONS OF THE RAT-BRAIN

被引:220
作者
ODONOHUE, TL [1 ]
MILLER, RL [1 ]
JACOBOWITZ, DM [1 ]
机构
[1] HOWARD UNIV,DEPT PHARMACOL & MED,DIV CLIN PHARMACOL,WASHINGTON,DC 20060
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0006-8993(79)90873-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A highly specific antibody to α-melanocyte stimulating hormone (α-MSH) was used to histochemically localize and biochemically identify and quantitate α-MSH immunoreactivity in nerve fibers and cell bodies of the rat brain. α-MSH-like immunoreactivity was contained in fibers throughout the brain. The distribution of α-MSH was determined by immunocytochemistry as well as by radioimmunoassay combined with microdissection techniques. High concentrations of α-MSH were contained in the nucleus interstitialis stria terminalis, the median eminence and the medial preoptic, anterior hypothalamic, periventricular, paraventricular, arcuate, dorsomedial, and posterior hypothalamic nuclei. Moderate α-MSH concentrations were noted in the amygdala, septum, central gray, dorsal raphe, and the nucleus tractus solitarius. Cell bodies containing α-MSH were observed only in the arcuate nucleus. The α-MSH-like compound in brain had similar immunochemical and electrophoretic properties of standard α-MSH but high pressure liquid chromatographic analysis demonstrated that the α-MSH-like immunoreactivity was comprised of one major and two minor components. The major immunoreactive peak had an identical retention time as α-MSH and therefore may be chemically identical to α-MSH. The similar retention times and immunoreactivity of the other two compounds suggest a similarity in size and structure to α-MSH. These observations demonstrate that fibers containing α-MSH emanate from the arcuate nucleus to innervate many other regions of the rat brain. © 1979.
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页码:101 / 123
页数:23
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