IMPROVED CALPAIN ASSAY USING FLUORESCEIN ISOTHIOCYANATE-LABELED CASEIN

被引：9

作者：

LONERGAN, SM ^{[1
]}

JOHNSON, MH ^{[1
]}

CALKINS, CR ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT ANIM SCI,LINCOLN,NE 68583

来源：

JOURNAL OF FOOD SCIENCE | 1995年 / 60卷 / 01期

关键词：

CALPAIN; ASSAY; FLUORESCEIN ISOTHIOCYANATE; CASEIN;

D O I：

10.1111/j.1365-2621.1995.tb05609.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A sensitive assay has been developed using a fluorescein isothiocyanate (FITC)-labeled casein substrate. Fluorescence of TCA-soluble FITC-labeled peptides was measured with an excitation wavelength of 490 nm and an emission wavelength of 513 nm. Fluorometric results in a time course assay correlated highly (r=0.985) with standard calpain assay (absorbance of TCA-soluble peptides at 278 nm) results. Fluorescence assay results correlated highly with calpain activity (r=0.997) in an activity titration assay and were substantially more sensitive than the standard method. Using FITC-labeled casein provides a simple, sensitive assay for mu- and m-calpain.

引用

页码：72 / &

共 10 条

[1] INTERACTION OF CHYMOTRYPSINOGENS WITH ALPHA-1-PROTEASE INHIBITOR [J].