FLOW CYTOMETRIC ESTIMATION OF NUCLEAR-DNA AMOUNT IN DIPLOID BANANAS (MUSA-ACUMINATA AND M-BALBISIANA)

被引：226
作者：
DOLEZEL, J
DOLEZELOVA, M
NOVAK, FJ
机构：
[1] Department of Plant Biotechnology, Institute of Experimental Botany, Olomouc, CZ-77200
[2] Plant Breeding Unit, Joint FAO/IAEA Programme, IAEA Laboratories, Seibersdorf
来源：
BIOLOGIA PLANTARUM | 1994年 / 36卷 / 03期
关键词：
D O I：
10.1007/BF02920930
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Cell nuclei were isolated from leaf tissues of wild banana (Musa balbisiana, M. acuminata ssp. banksii and M. acuminata ssp. errans) and of the two vegetative clones of diploid cultivar ''Pisang Mas''. Relative fluorescence intensity was measured on propidium iodide-stained nuclei by flow cytometry. Nuclei isolated from Gylcine max with known nuclear genome size were used as internal standard to determine nuclear DNA content of Musa in absolute units. The results of the study showed that the size of nuclear genome of Musa is smaller than previously estimated. In general, it is smaller in comparison with many other angiosperms. Furthermore, it was found that nuclear DNA content of M. balbisiana (genome BB) is significantly lower than that of M. acuminata subspecies and cultivars (genome AA). This finding should permit estimation of genome composition in triploid Musa clones with expected hybrid composition. Flow cytometry is proposed as a useful technique with potential applications in taxonomy, breeding and biotechnology of Musa.
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页码：351 / 357
页数：7
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