DETERMINATION OF MURAMIC ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY PLASMA SPRAY MASS-SPECTROMETRY

被引：7
作者：
ELMROTH, I
LARSSON, L
WESTERDAHL, G
ODHAM, G
机构：
[1] UNIV LUND,DEPT MED MICROBIOL,S-22362 LUND,SWEDEN
[2] UNIV LUND,DEPT ECOL,DIV CHEM ECOL,S-22362 LUND,SWEDEN
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 598卷 / 01期
关键词：
D O I：
10.1016/0021-9673(92)85112-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic-plasma spray mass spectrometric method was developed for the determination of muramic acid, a unique compound present in bacterial peptidoglycan. The method included hydrolysis of bacterial samples by hydrochloric acid, purification using a disposable extraction column, and separation using cation-exchange chromatography with a mobile phase composed of ammonium acetate-trifluoroacetic acid-water. Muramic acid was detected in cells of Bacillus subtilis; the presence of yeast cells (Saccharomyces cerevisiae) in excess amounts did not interfere with the analyses. In contrast to currently applied liquid and gas chromatographic methods, the described method permits highly selective determination of underivatized muramic acid, and may therefore be useful for detecting bacteria and bacterial cell debris in complex biological matrices.
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页码：43 / 50
页数：8
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