DEOXYRIBONUCLEASE ACTIVITY FOUND IN EPSTEIN-BARR VIRUS PRODUCING LYMPHOBLASTOID-CELLS

被引：44

作者：

CLOUGH, W ^{[1
]}

机构：

[1] UNIV SO CALIF, DIV MOLEC BIOL, LOS ANGELES, CA 90007 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 21期

关键词：

D O I：

10.1021/bi00588a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A DNase activity from Epstein-Barr (EB) virus producer [human] lymphocyte cell lines which is correlated with viral production and which is not present in virus nonproducer or negative lymphocyte cell lines was purified 220-fold with 20% recovery and characterized. This nuclease copurifies through DEAE-cellulose column chromatography with the EB virus-induced DNA polymerase in EB virus producer cells, which was recently reported by this laboratory, but elutes as a separate peak of activity upon phosphocellulose chromatography. This nuclease activity has a sedimentation coefficient of 4.0 S, a strong divalent cation requirement, an alkaline pH optimum and the ability to utilize native and denatured lymphocyte DNA as substrate, reducing both to monophosphonucleosides.

引用

页码：4517 / 4521

页数：5

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