C-1-C-X REVISITED - INTRAMOLECULAR SYNERGISM IN A CELLULASE

被引：143
作者：
DIN, N
DAMUDE, HG
GILKES, NR
MILLER, RC
WARREN, RAJ
KILBURN, DG
机构：
[1] UNIV BRITISH COLUMBIA, DEPT MICROBIOL & IMMUNOL, VANCOUVER V6T 1Z3, BC, CANADA
[2] UNIV BRITISH COLUMBIA, BIOTECHNOL LAB, VANCOUVER V6T 1Z3, BC, CANADA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 24期
关键词：
D O I：
10.1073/pnas.91.24.11383
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Endoglucanase A (CenA) from the bacterium Cellulomonas fimi is composed of a catalytic domain and a nonhydrolytic cellulose-binding domain that can function independently. The individual domains interact synergistically in the disruption and hydrolysis of cellulose fibers. This intramolecular synergism is distinct from the well known intermolecular synergism between individual cellulases. The catalytic domain corresponds to the hydrolytic C-X system and the cellulose-binding domain corresponds to the nonhydrolytic C-1 system postulated by Reese et al. [Reese, E. T., Sui, R. G. H. and Levinson, H. S. (1950) J. Bacteriol. 59, 485-497] to be required for the hydrolysis of cellulose.
引用
收藏
页码：11383 / 11387
页数：5
相关论文
共 26 条
[1]  
Coughlan M. P., 1988, Biochemistry and genetics of cellulose degradation, P11
[2]   NON-HYDROLYTIC DISRUPTION OF CELLULOSE FIBERS BY THE BINDING DOMAIN OF A BACTERIAL CELLULASE [J].
DIN, N ;
GILKES, NR ;
TEKANT, B ;
MILLER, RC ;
WARREN, AJ ;
KILBURN, DG .
BIO-TECHNOLOGY, 1991, 9 (11) :1096-1099
[3]   THE CELLULOSE-BINDING DOMAIN OF ENDOGLUCANASE-A (CENA) FROM CELLULOMONAS-FIMI - EVIDENCE FOR THE INVOLVEMENT OF TRYPTOPHAN RESIDUES IN BINDING [J].
DIN, N ;
FORSYTHE, IJ ;
BURTNICK, LD ;
GILKES, NR ;
MILLER, RC ;
WARREN, RAJ ;
KILBURN, DG .
MOLECULAR MICROBIOLOGY, 1994, 11 (04) :747-755
[4]   THE 1.4-BETA-GLUCAN CELLOBIOHYDROLASES OF TRICHODERMA-REESEI QM-9414 - A NEW TYPE OF CELLULOLYTIC SYNERGISM [J].
FAGERSTAM, LG ;
PETTERSSON, LG .
FEBS LETTERS, 1980, 119 (01) :97-100
[5]   CLONING OF THE THERMOMONOSPORA-FUSCA ENDOGLUCANASE E2 GENE IN STREPTOMYCES-LIVIDANS - AFFINITY PURIFICATION AND FUNCTIONAL DOMAINS OF THE CLONED GENE-PRODUCT [J].
GHANGAS, GS ;
WILSON, DB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (10) :2521-2526
[6]  
GILKES NR, 1992, J BIOL CHEM, V267, P6743
[7]   DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES [J].
GILKES, NR ;
HENRISSAT, B ;
KILBURN, DG ;
MILLER, RC ;
WARREN, RAJ .
MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :303-315
[8]   VISUALIZATION OF THE ADSORPTION OF A BACTERIAL ENDO-BETA-1,4-GLUCANASE AND ITS ISOLATED CELLULOSE-BINDING DOMAIN TO CRYSTALLINE CELLULOSE [J].
GILKES, NR ;
KILBURN, DG ;
MILLER, RC ;
WARREN, RAJ ;
SUGIYAMA, J ;
CHANZY, H ;
HENRISSAT, B .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1993, 15 (06) :347-351
[9]  
GILKES NR, 1988, J BIOL CHEM, V263, P10401
[10]   ANALYSIS OF FUNCTIONAL DOMAINS OF ENDOGLUCANASES FROM CLOSTRIDIUM-CELLULOVORANS BY GENE CLONING, NUCLEOTIDE SEQUENCING AND CHIMERIC PROTEIN CONSTRUCTION [J].
HAMAMOTO, T ;
FOONG, F ;
SHOSEYOV, O ;
DOI, RH .
MOLECULAR AND GENERAL GENETICS, 1992, 231 (03) :472-479
123