AMPEROMETRIC GLUCOSE BIOSENSOR BASED ON AN ELECTROCATALYTICALLY BULK-MODIFIED EPOXY-GRAPHITE BIOCOMPOSITE

被引：39

作者：

CESPEDES, F ^{[1
]}

MARTINEZFABREGAS, E ^{[1
]}

ALEGRET, S ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA,DEPT QUIM,SENSORS & BIOSENSORS GRP,E-08193 BARCELONA,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 284卷 / 01期

关键词：

AMPEROMETRY; BIOSENSORS; CATALYTIC METHODS; BIOCOMPOSITES; GLUCOSE BIOSENSOR; EPOXY-GRAPHITE BIOCOMPOSITE;

D O I：

10.1016/0003-2670(93)80003-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCI). The biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface.

引用

页码：21 / 26

页数：6

共 10 条

[1] BIOSENSORS BASED ON CONDUCTING FILLED POLYMER ALL-SOLID-STATE PVC MATRIX MEMBRANE ELECTRODES [J].

ALEGRET, S ;

MARTINEZFABREGAS, E .

BIOSENSORS, 1989, 4 (05) :287-297

[2] AMPEROMETRIC ENZYMATIC GLUCOSE ELECTRODE BASED ON AN EPOXY GRAPHITE COMPOSITE [J].

CESPEDES, F ;

MARTINEZFABREGAS, E ;

BARTROLI, J ;

ALEGRET, S .

ANALYTICA CHIMICA ACTA, 1993, 273 (1-2) :409-417

[3] ELIMINATION OF INTERFERENCES IN FLOW-INJECTION AMPEROMETRIC DETERMINATION OF GLUCOSE IN BLOOD-SERUM USING IMMOBILIZED GLUCOSE-OXIDASE [J].

MATUSZEWSKI, W ;

TROJANOWICZ, M ;

LEWENSTAM, A .

ELECTROANALYSIS, 1990, 2 (08) :607-615

[4] CHEMICALLY MODIFIED CARBON PASTE AND CARBON COMPOSITE ELECTRODES [J].

STARA, V ;

KOPANICA, M .

ELECTROANALYSIS, 1989, 1 (03) :251-256

[5] MODIFIED ELECTRODES FOR ELECTROCHEMICAL SENSORS [J].

WANG, J .

ELECTROANALYSIS, 1991, 3 (4-5) :255-259

[6] POLISHABLE AND ROBUST BIOLOGICAL ELECTRODE SURFACES [J].

WANG, J ;

VARUGHESE, K .

ANALYTICAL CHEMISTRY, 1990, 62 (03) :318-320

[7] RENEWABLE ALCOHOL BIOSENSORS BASED ON ALCOHOL-DEHYDROGENASE NICOTINAMIDE-ADENINE-DINUCLEOTIDE GRAPHITE EPOXY ELECTRODES [J].

WANG, J ;

GONZALEZROMERO, E ;

OZSOZ, M .

ELECTROANALYSIS, 1992, 4 (05) :539-544

[8] A POLISHABLE AMPEROMETRIC BIOSENSOR FOR BILIRUBIN [J].

WANG, J ;

OZSOZ, M .

ELECTROANALYSIS, 1990, 2 (08) :647-650

[9] BULK MODIFIED ENZYME ELECTRODES FOR REAGENTLESS DETECTION OF PEROXIDES [J].

WOLLENBERGER, U ;

WANG, J ;

OZSOZ, M ;

GONZALEZROMERO, E ;

SCHELLER, F .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1991, 26 (02) :287-296

[10]

YANG XR, 1989, MIKROCHIM ACTA, V1, P9

← 1 →