DIRECT RECOVERY OF INTRACELLULAR PROTEINS FROM CANDIDA-UTILIS USING REVERSE MICELLES IN COMBINATION WITH A REDUCING AGENT

被引：4

作者：

HAN, DH ^{[1
]}

LEE, SY ^{[1
]}

HONG, WH ^{[1
]}

机构：

[1] KOREA ADV INST SCI & TECHNOL,BIOPROC ENGN RES CTR,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 08期

关键词：

D O I：

10.1007/BF00156326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intracellular proteins were directly extracted from Candida utilis using reverse micelles in combination with a reducing agent. Extraction at pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabilization and protein solubilization. Stripping at pH 7 in the presence of 0.5 M KCl resulted in the efficient recovery of solubilized proteins.

引用

页码：545 / 550

页数：6

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