ANTIBODY-RESPONSE TO THE M2-PROTEIN OF INFLUENZA A-VIRUS EXPRESSED IN INSECT CELLS

被引：112
作者：
BLACK, RA
ROTA, PA
GORODKOVA, N
KLENK, HD
KENDAL, AP
机构：
[1] ACAD SCI,INST MICROBIOL,RIGA,LATVIA
[2] UNIV MARBURG,INST VIROL,W-3550 MARBURG,GERMANY
来源：
JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷
关键词：
D O I：
10.1099/0022-1317-74-1-143
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS-polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses.
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页码：143 / 146
页数：4
相关论文
共 26 条
  • [1] GENETIC-BASIS OF RESISTANCE TO RIMANTADINE EMERGING DURING TREATMENT OF INFLUENZA-VIRUS INFECTION
    BELSHE, RB
    SMITH, MH
    HALL, CB
    BETTS, R
    HAY, AJ
    [J]. JOURNAL OF VIROLOGY, 1988, 62 (05) : 1508 - 1512
  • [2] BELSHE RB, 1989, J RESPIR DIS S, V10, pS52
  • [3] REGULATION OF PH BY THE M2 PROTEIN OF INFLUENZA-A VIRUSES
    CIAMPOR, F
    THOMPSON, CA
    GRAMBAS, S
    HAY, AJ
    [J]. VIRUS RESEARCH, 1992, 22 (03) : 247 - 258
  • [4] EVIDENCE THAT THE AMANTADINE-INDUCED, M2-MEDIATED CONVERSION OF INFLUENZA-A VIRUS HEMAGGLUTININ TO THE LOW PH CONFORMATION OCCURS IN AN ACIDIC TRANSGOLGI COMPARTMENT
    CIAMPOR, F
    BAYLEY, PM
    NERMUT, MV
    HIRST, EMA
    SUGRUE, RJ
    HAY, AJ
    [J]. VIROLOGY, 1992, 188 (01) : 14 - 24
  • [5] COMPARATIVE-STUDIES OF WILD-TYPE AND COLD-MUTANT (TEMPERATURE-SENSITIVE) INFLUENZA-VIRUS - DETECTION OF MUTATIONS IN ALL GENES OF THE A/ANN ARBOR/6/60(H2N2) MUTANT VACCINE DONOR STRAIN
    COX, NJ
    KITAME, F
    KLIMOV, A
    KOENNECKE, I
    KENDAL, AP
    [J]. MICROBIAL PATHOGENESIS, 1986, 1 (04) : 387 - 397
  • [6] IDENTIFICATION OF VIRAL MOLECULES RECOGNIZED BY INFLUENZA-SPECIFIC HUMAN CYTOTOXIC LYMPHOCYTES-T
    GOTCH, F
    MCMICHAEL, A
    SMITH, G
    MOSS, B
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (02) : 408 - 416
  • [7] MOLECULAR-BASIS OF RESISTANCE OF INFLUENZA-A VIRUSES TO AMANTADINE
    HAY, AJ
    ZAMBON, MC
    WOLSTENHOLME, AJ
    SKEHEL, JJ
    SMITH, MH
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1986, 18 : 19 - 29
  • [8] HAY AJ, 1989, CONCEPTS VIRAL PATHO, V3, P561
  • [9] INFLUENZA VIRUS-M2 INTEGRAL MEMBRANE-PROTEIN IS A HOMOTETRAMER STABILIZED BY FORMATION OF DISULFIDE BONDS
    HOLSINGER, LJ
    LAMB, RA
    [J]. VIROLOGY, 1991, 183 (01) : 32 - 43
  • [10] EVOLUTIONARY ANALYSIS OF THE INFLUENZA-A VIRUS M-GENE WITH COMPARISON OF THE M1 AND M2 PROTEINS
    ITO, T
    GORMAN, OT
    KAWAOKA, Y
    BEAN, WJ
    WEBSTER, RG
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (10) : 5491 - 5498
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