RAT-BRAIN EXPRESSES AN ALTERNATIVELY SPLICED FORM OF THE DIHYDROPYRIDINE-SENSITIVE L-TYPE CALCIUM-CHANNEL ALPHA-2 SUBUNIT

被引：109

作者：

KIM, HL ^{[1
]}

KIM, H ^{[1
]}

LEE, P ^{[1
]}

KING, RG ^{[1
]}

CHIN, H ^{[1
]}

机构：

[1] NINCDS,MOLEC BIOL LAB,BLDG 36,3D-02,BETHESDA,MD 20892

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 08期

关键词：

ION CHANNEL; PRIMARY STRUCTURE; INSITU HYBRIDIZATION; RNA SPLICING;

D O I：

10.1073/pnas.89.8.3251

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have cloned and sequenced a cDNA (rB-alpha-2) encoding an alpha-2 subunit of the voltage-sensitive L-type calcium channel (dihydropyridine receptor) of rat brain. The cDNA (3823 base pairs) encodes a protein of 1091 amino acids with a M(r) of 123,822. The deduced amino acid sequence of rB-alpha-2 cDNA is highly similar (95% amino acid identity) to that of rabbit skeletal muscle alpha-2 subunit. The rB-alpha-2 protein is distinct from the previously cloned skeletal muscle alpha-2-subunit protein [Ellis, S. B., Williams, M. E., Ways, N. R., Brenner, R., Sharp, A. H., Leung, A. T., Campbell, K. P., McKenna, E., Koch, W., Hui, A., Schwartz, A. & Harpold, M. M. (1988) Science 241, 1661-1664] because it contains an insertion of 7 amino acid residues and a deletion of a 19-amino acid segment between putative transmembrane domains 1 and 2. We show that the rB-alpha-2 and skeletal muscle alpha-2-subunit transcripts are the variants produced by alternative splicing of the primary transcript and that they are differentially expressed in brain and skeletal muscle, respectively.

引用

页码：3251 / 3255

页数：5

共 26 条

[1] SUBUNIT STRUCTURE AND LOCALIZATION OF DIHYDROPYRIDINE-SENSITIVE CALCIUM CHANNELS IN MAMMALIAN BRAIN, SPINAL-CORD, AND RETINA [J].