TRANSFORMATION-DEFECTIVE V-SKI INDUCES MYOD AND MYOGENIN EXPRESSION BUT NOT MYOTUBE FORMATION

被引：61
作者：
COLMENARES, C
TEUMER, JK
STAVNEZER, E
机构：
[1] UNIV CINCINNATI,MED CTR,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267
[2] HARVARD UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS,BOSTON,MA 02115
来源：
MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 02期
关键词：
D O I：
10.1128/MCB.11.2.1167
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation.
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页码：1167 / 1170
页数：4
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