PURIFICATION AND CHARACTERIZATION OF A CYTOSOLIC PHOSPHOINOSITIDE-PHOSPHOLIPASE-C (GAMMA-2-TYPE) FROM HUMAN PLATELETS

被引：31
作者：
BANNO, Y
YU, AM
NAKASHIMA, T
HOMMA, Y
TAKENAWA, T
NOZAWA, Y
机构：
[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,TSUKASAMACHI 40,GIFU 500,JAPAN
[2] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL,DEPT PHARMACOL,ITABASHI KU,TOKYO 173,JAPAN
[3] GIFU UNIV,SCH MED,DEPT NEUROSURG,GIFU 500,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)92035-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A human platelet cytosolic phosphoinositide-specific phospholipase C, one of four PLC activity peaks separated by column chromatographies, designated as cPLC-I, was purified to homogeneity. The cPLC-I exhibited an apparent Mr of 145 kDa by SDS-polyacrylamide gel electrophoresis and was immunologically identified to be PLC-γ2. It hydrolyzed PI and PIP2 at optimum pH of 5.5-6.0. Deoxycholate and cholate inhibited the enzyme activity to hydrolyze two substrates. Calcium was required to obtain the maximal activity for PI- and PIP2-hydrolysis at concentration of 10-3 M and 10-5 M, respectively. Hg2+ (1 μM) inhibited strongly the enzyme activity. © 1990.
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页码：396 / 401
页数：6
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