CHROMATOGRAPHIC PROPERTIES OF SEPHACRYL S-300 SUPERFINE

被引：12

作者：

JOHANSSON, I

LUNDGREN, H

机构：

[1] Pharmacia Fine Chemicals AB, Box 175

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 01期

关键词：

colostrum; gel filtration; Sephacryl; serum; thyroglobulin;

D O I：

10.1016/0165-022X(79)90044-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sephacryl S-300 Superfine is a new gel filtration medium that consists of allydextran crosslinked with N,N′-methylenebisacrylamide. It was shown to have a sepration range for proteins that extends to 1-2 · 106 dalton. The gel filtration properties were characterized by fractionation of human serum and of bovine colostrum. Thyroglobulin from pig could be isolated in one step in a very pure form. The flow properties were characterized by the relationship between flow rate and pressure drop. It was shown that Sephacryl has very good flow properties in comparison with Sephadex G-200 and that it can be used in large-scale columns. © 1979.

引用

页码：37 / 44

页数：8

共 11 条

[1] USE OF ACRYLAMIDE-AGAROSE BEADS IN GEL-FILTRATION OF PROTEINS [J].