A PSEUDOSUBSTRATE PEPTIDE INHIBITS PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION IN PERMEABILIZED RAT-1 CELLS

被引：26
|
作者：
EICHHOLTZ, T [1 ]
ALBLAS, J [1 ]
VANOVERVELD, M [1 ]
MOOLENAAR, W [1 ]
PLOEGH, H [1 ]
机构：
[1] NETHERLANDS CANC INST,DEPT CELLULAR BIOCHEM,PLESMANLAAN 121,1066 CX AMSTERDAM,NETHERLANDS
来源：
FEBS LETTERS | 1990年 / 261卷 / 01期
关键词：
(Rat-1 cell); Protein kinase C; Protein; 80; kDa; Pseudosubstrate peptide; Signal transduction;
D O I：
10.1016/0014-5793(90)80657-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digiton-in-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 μM, while a control peptide had no effect. The results indicate that this semi-intact cell system can be used in combination with the inhibitory pseudosubstrate peptide to study the involvement of PKC in cellular processes. © 1990.
引用
收藏
页码：147 / 150
页数：4
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