A PSEUDOSUBSTRATE PEPTIDE INHIBITS PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION IN PERMEABILIZED RAT-1 CELLS

被引：26

作者：

EICHHOLTZ, T ^{[1
]}

ALBLAS, J ^{[1
]}

VANOVERVELD, M ^{[1
]}

MOOLENAAR, W ^{[1
]}

PLOEGH, H ^{[1
]}

机构：

[1] NETHERLANDS CANC INST,DEPT CELLULAR BIOCHEM,PLESMANLAAN 121,1066 CX AMSTERDAM,NETHERLANDS

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

(Rat-1 cell); Protein kinase C; Protein; 80; kDa; Pseudosubstrate peptide; Signal transduction;

D O I：

10.1016/0014-5793(90)80657-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digiton-in-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 μM, while a control peptide had no effect. The results indicate that this semi-intact cell system can be used in combination with the inhibitory pseudosubstrate peptide to study the involvement of PKC in cellular processes. © 1990.

引用

页码：147 / 150

页数：4

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