A PSEUDOSUBSTRATE PEPTIDE INHIBITS PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION IN PERMEABILIZED RAT-1 CELLS

被引：26
|
作者：
EICHHOLTZ, T [1 ]
ALBLAS, J [1 ]
VANOVERVELD, M [1 ]
MOOLENAAR, W [1 ]
PLOEGH, H [1 ]
机构：
[1] NETHERLANDS CANC INST,DEPT CELLULAR BIOCHEM,PLESMANLAAN 121,1066 CX AMSTERDAM,NETHERLANDS
来源：
FEBS LETTERS | 1990年 / 261卷 / 01期
关键词：
(Rat-1 cell); Protein kinase C; Protein; 80; kDa; Pseudosubstrate peptide; Signal transduction;
D O I：
10.1016/0014-5793(90)80657-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digiton-in-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 μM, while a control peptide had no effect. The results indicate that this semi-intact cell system can be used in combination with the inhibitory pseudosubstrate peptide to study the involvement of PKC in cellular processes. © 1990.
引用
收藏
页码：147 / 150
页数：4
相关论文
共 50 条
  • [1] PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION OF RAT INTESTINAL MICROVILLUS MEMBRANES
    HENDERSON, LS
    NASELSKY, D
    FONDACARO, JD
    GASTROENTEROLOGY, 1985, 88 (05) : 1417 - 1417
  • [2] A ROLE FOR PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION IN ELICITING GLUCAGON DESENSITIZATION IN RAT HEPATOCYTES
    SAVAGE, A
    ZENG, L
    HOUSLAY, MD
    BIOCHEMICAL JOURNAL, 1995, 307 : 281 - 285
  • [3] ENHANCEMENT OF PROTEIN-KINASE C-MEDIATED EGF RECEPTOR PHOSPHORYLATION BY AURANOFIN
    FROSCIO, M
    MURRAY, AW
    HURST, NP
    SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 1988, 17 (04) : 281 - 285
  • [4] BILIRUBIN INHIBITS PROTEIN-KINASE-C ACTIVITY AND PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION OF ENDOGENOUS SUBSTRATES IN HUMAN SKIN FIBROBLASTS
    AMIT, Y
    BONEH, A
    CLINICA CHIMICA ACTA, 1993, 223 (1-2) : 103 - 111
  • [5] CYCLIC-GMP INHIBITS PROTEIN-KINASE C-MEDIATED SECRETION IN RAT PANCREATIC ACINI
    ROGERS, J
    HUGHES, RG
    MATTHEWS, EK
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1988, 263 (08) : 3713 - 3719
  • [6] PROTEIN-KINASE C-MEDIATED PROTEIN-PHOSPHORYLATION IN ELECTRICALLY PERMEABILISED ISLETS OF LANGERHANS
    JONES, PM
    PERSAUD, SJ
    HOWELL, SL
    DIABETOLOGIA, 1989, 32 (07) : A500 - A500
  • [7] PHOSPHORYLATION OF VIMENTIN IS AN INTERMEDIATE STEP IN PROTEIN-KINASE C-MEDIATED GLYCOCONJUGATE SECRETION
    BERTRAND, F
    VEISSIERE, D
    HERMELIN, B
    PAUL, A
    CAPEAU, J
    PICARD, J
    CHERQUI, G
    AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (03): : C611 - C621
  • [8] Protein kinase C-mediated phosphorylation of Kvβ in adult rat brain
    Wang, XT
    Belkowski, SM
    Knowles, H
    Chandramouly, AB
    Downen, M
    Prystowsky, MB
    FASEB JOURNAL, 2000, 14 (04): : A108 - A108
  • [9] EVIDENCE FOR A SINGLE PROTEIN-KINASE C-MEDIATED PHOSPHORYLATION SITE IN RAT-BRAIN PROTEIN B-50
    COGGINS, PJ
    ZWIERS, H
    JOURNAL OF NEUROCHEMISTRY, 1989, 53 (06) : 1895 - 1901
  • [10] PROTEIN-KINASE C-MEDIATED CONTRACTILE RESPONSE OF THE RAT VAS-DEFERENS
    ABRAHAM, ST
    RICE, PJ
    EUROPEAN JOURNAL OF PHARMACOLOGY, 1992, 218 (2-3) : 243 - 249
12345