PYROPHOSPHATE-DEPENDENT 6-PHOSPHOFRUCTOKINASE, A NEW GLYCOLYTIC ENZYME IN PINEAPPLE LEAVES

被引：121
作者：
CARNAL, NW
BLACK, CC
机构：
[1] Biochemistry and Botany Department, University of Georgia, Athens
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 01期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/0006-291X(79)90376-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pineapple leaves contain a pyrophosphate-dependent 6-phosphofructokinase which has been partially purified and characterized. In crude extracts the pyrophosphate-dependent activity is 10 to 20-fold higher than the ATP-dependent activity. The partially purified activity is near 2.5 μmol Fru-1,6-P2 formed/min/mg protein. In the reaction 1 Fru-1,6-P2 is formed per 1 pyrophosphate consumed. The enzyme exhibits a pH optimum of 8.0 and the activity is stimulated by Mg++. The discovery of a pyrophosphate-dependent 6-phosphofructokinase in pineapple leaves indicates pyrophosphate can serve as an energy source for synthetic reactions in pineapple and perhaps in other plants as well. © 1979.
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页码：20 / 26
页数：7
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