ABSENCE OF EPIZOOTIC HEMATOPOIETIC NECROSIS VIRUS (EHNv) IN DISEASED RAINBOW TROUT (Oncorhynchus mykiss) FROM FISH FARMS IN THE PERUVIAN HIGHLANDS

被引:0
作者
Bautista D, Karen [1 ]
Manchego S, Alberto [1 ,2 ]
Castro S, Gina [1 ]
Sandoval C, Nieves
机构
[1] Univ Nacl Mayor San Marcos, Fac Med Vet, Lab Microbiol & Parasitol Vet, Lima, Peru
[2] Univ Nacl Mayor San Marcos, Fac Med Vet, Lab Histol Embriol & Patol Vet, Lima, Peru
来源
REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU | 2018年 / 29卷 / 03期
关键词
rainbow trout; epizootic hematopoietic necrosis virus; PCR; bacterial isolation;
D O I
10.15381/rivep.v29i3.14841
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The objective of the present study was to monitor the detection of the Epizootic Hematopoietic Necrosis virus (EHNv) in trouts (Oncorhynchus mykiss) in fish farms of the highlands of Peru. Samples were taken from 111 diseased fish (liver, spleen, anterior kidney) from fish farms in the regions of Ancash, Junin and Huancavelica, Peru. The tissue samples were processed immediately for bacterial isolation and another group of samples were stored at -196 degrees C for PCR. The DNA of each tissue was extracted by the Trizol method and then purified with silica membranes (PureLink Genomic DNA kit). The PCR was performed using the MCP-1 primers specific for the EHNv, following the methodology proposed by the OIE and with the commercial kit VetPCR (TM) EHNV Detection for confirmation. Bacterial isolation was carried out by culturing on TSA and AnackerOrdal agar (Cytophaga agar) of each tissue, and the identification by Gram stain and bacterial biochemistry. The EHNv DNA was not detected indicating that the prevalence of the virus is less than 5% or it is not present in the sampled fish farms. Yersinia ruckeri and Aeromonas spp were isolated in all the samples, indicating that the cause of the disease and mortality present in the fish farms was due to the infection with these bacteria.
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页码:957 / 963
页数:7
相关论文
共 15 条
  • [1] Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish
    Beaz-Hidalgo, Roxana
    Alperi, Anabel
    Bujan, Noemi
    Romalde, Jesus L.
    Jose Figueras, Maria
    [J]. SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (03) : 149 - 153
  • [2] Bravo S, 2004, B EUR ASSOC FISH PAT, V24, P104
  • [3] Chinchar VG, 2009, CURR TOP MICROBIOL, V328, P123
  • [4] FAO, 2016, EST MUND PESC AC 201
  • [5] Kleeberg F, 2012, PESQUERIA ACUICULTUR
  • [6] OIE, 2012, MAN DIAGN TEST AQ AN
  • [7] [OIE] Organizacion Mundial de Sanidad Animal, 2017, COD SAN AN AC
  • [8] Ostrander GK., 2000, LAB FISH
  • [9] Pathology of epizootic haematopoietic necrosis virus (EHNV) infection in rainbow trout (Oncorhynchus mykiss walbaum) and redfin perch (Perca fluviatilis L)
    Reddacliff, LA
    Whittington, RJ
    [J]. JOURNAL OF COMPARATIVE PATHOLOGY, 1996, 115 (02) : 103 - 115
  • [10] Roberts RJ, 1980, ENFERMEDADES TRUCHA