BETA-GUANIDINOPROPIONATE AND PHOSPHORYLATED BETA-GUANIDINOPROPIONATE AS SUBSTRATES FOR CREATINE-KINASE

被引：58
作者：
CHEVLI, R
FITCH, CD
机构：
[1] Department of Internal Medicine, St. Louis University School of Medicine, St. Louis
来源：
BIOCHEMICAL MEDICINE | 1979年 / 21卷 / 02期
关键词：
D O I：
10.1016/0006-2944(79)90068-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rabbit muscle creatine kinase catalyzes transphosphorylation between ATP and β-guanidinopropionate at pH 9 and 23°C with a Km for β-guanidinopropionate of 50 mm and a Vmax of 0.21 μmole/min/mg of creatine kinase. The comparable values for the phosphorylation of creatine are 16.7 for Km and 75.1 for Vmax. In the reverse direction at pH 7.05 and 23°C, phosphorylated β-guanidinopropionate has a Km of 4.9 and a Vmax of 0.22 in comparison to a Km of 2.2 and a Vmax of 231 for phosphocreatine. © 1979.
引用
收藏
页码：162 / 167
页数：6
相关论文
共 19 条
[1]   POSSIBLE ROLE OF MITOCHONDRIAL BOUND CREATINE KINASE IN REGULATION OF MITOCHONDRIAL RESPIRATION [J].
BESSMAN, SP ;
FONYO, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 22 (05) :597-+
[2]  
DIEHL JF, 1959, P SOC EXP BIOL MED, V100, P657, DOI 10.3181/00379727-100-24734
[3]   BIOCHEMISTRY OF THE PHOSPHAGENS AND RELATED GUANIDINES [J].
ENNOR, AH ;
MORRISON, JF .
PHYSIOLOGICAL REVIEWS, 1958, 38 (04) :631-674
[4]  
Fitch C. D., 1977, PATHOGENESIS HUMAN M, P328
[5]   SUSTAINED ISOMETRIC CONTRACTION OF SKELETAL-MUSCLE DEPLETED OF PHOSPHOCREATINE [J].
FITCH, CD ;
CHEVLI, R ;
PETROFSKY, JS ;
KOPP, SJ .
LIFE SCIENCES, 1978, 23 (12) :1285-1291
[6]  
FITCH CD, 1968, J BIOL CHEM, V243, P2024
[7]  
FITCH CD, 1974, J BIOL CHEM, V249, P1060
[8]   CREATINE METABOLISM IN SKELETAL MUSCLE .2. CREATINE KINETICS IN MAN [J].
FITCH, CD ;
LUCY, DD ;
BORNHOFE.JH ;
DALRYMPL.GL .
NEUROLOGY, 1968, 18 (1P1) :32-&
[9]   PHOSPHORYLATED BETA-GUANIDINOPROPIONATE AS A SUBSTITUTE FOR PHOSPHOCREATINE IN RAT MUSCLE [J].
FITCH, CD ;
JELLINEK, M ;
FITTS, RH ;
BALDWIN, KM ;
HOLLOSZY, JO .
AMERICAN JOURNAL OF PHYSIOLOGY, 1975, 228 (04) :1123-1125
[10]   MEASUREMENT OF BETA-GUANIDINOPROPIONATE AND PHOSPHORYLATED BETA-GUANIDINOPROPIONATE IN TISSUES [J].
FITCH, CD ;
CHEVLI, R .
ANALYTICAL BIOCHEMISTRY, 1975, 68 (01) :196-201
12