DIFFERENTIAL EXPRESSION OF SODIUM-CHANNELS AND NICOTINIC ACETYLCHOLINE-RECEPTOR CHANNELS IN NNR VARIANTS OF THE PC12 PHEOCHROMOCYTOMA CELL-LINE

被引:0
作者
FANGER, GR
BRENNAN, C
HENDERSON, LP
GARDNER, PD
MAUE, RA
机构
[1] DARTMOUTH COLL,SCH MED,DEPT BIOCHEM,HANOVER,NH 03755
[2] DARTMOUTH COLL,SCH MED,DEPT PHYSIOL,HANOVER,NH 03755
[3] UNIV TEXAS,HLTH SCI CTR,CTR MOLEC MED,INST BIOTECHNOL,SAN ANTONIO,TX 78245
关键词
SODIUM CHANNELS; ACETYLCHOLINE RECEPTOR CHANNELS; PATCH CLAMPING; GENE EXPRESSION; PC12; CELLS; REGULATION;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An important component of neuronal differentiation is the tightly controlled expression of a spectrum of ion channel proteins. Ion channels play a critical role in the generation and propagation of action potentials as well as in the cellular response to neurotransmitters, and thus are central in the transfer and integration of information in the nervous system. A model system amenable to analysis of ion channel expression and neuronal differentiation is the rat pheochromocytoma (PC12) cell line. Here, we have used electrophysiological and molecular biological approaches to analyze the expression of voltage-dependent sodium (Na) channels and nicotinic acetylcholine receptors (nAChR) in mutagenized variants (nnr cells) of the PC12 cell line. Our data reveal striking differences in the expression of these channels when compared to wild-type PC12 cells. Even in the absence of nerve growth factor (NGF), nnr cells express functional Na channels and Na channel mRNA at levels exceeding those in wild-type PC12 cells differentiated with NGF. In contrast, acetylcholine-induced currents were evident in only a small proportion of cells, presumably due to the altered pattern of expression of mRNAs encoding individual nAChR subunits. The altered ion channel expression in these Variants provides an avenue for analyzing Na channel and nAChR channel function, as well as for identifying mechanisms governing their expression.
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页码:71 / 80
页数:10
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