ISOLATION, CHARACTERIZATION, AND NUCLEOTIDE-SEQUENCE OF IS1202, AN INSERTION-SEQUENCE OF STREPTOCOCCUS-PNEUMONIAE

被引:37
作者
MORONA, JK
GUIDOLIN, A
MORONA, R
HANSMAN, D
PATON, JC
机构
[1] WOMENS & CHILDRENS HOSP, DEPT MICROBIOL, ADELAIDE, SA 5006, AUSTRALIA
[2] UNIV ADELAIDE, DEPT MICROBIOL & IMMUNOL, ADELAIDE, SA 5005, AUSTRALIA
关键词
D O I
10.1128/jb.176.14.4437-4443.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A comparative hybridization protocol was used to isolate a small segment of DNA present in the Streptococcus pneumoniae type 19F strain SSZ but absent from strain Rx1, a nonencapsulated derivative of the type 2 strain D39. This segment of DNA is a 1,747-bp insertion sequence, designated IS1202, flanked by 23-bp imperfect inverted repeats and containing a single open reading frame sufficient to encode a 54.4-kDa polypeptide. A 27-bp target sequence is duplicated at either end of the element. IS1202 is not related to any of the currently known insertion elements and is the first reported for S. pneumoniae. Although found predominantly in type 19F strains in up to five copies, it has also been shown to be present in the chromosomes of pneumococci belonging to other serotypes. One of the four IS1202 copies in the encapsulated strain SSZ is located 1,009 bp downstream of the dexB gene, and transformation studies reveal that it is also closely linked to the type 19F capsular polysaccharide synthesis (cps) locus.
引用
收藏
页码:4437 / 4443
页数:7
相关论文
共 29 条
  • [21] Morelle G, 1989, FOCUS, V11, P7
  • [22] INFECTIONS CAUSED BY STREPTOCOCCUS-PNEUMONIAE - CLINICAL SPECTRUM, PATHOGENESIS, IMMUNITY, AND TREATMENT
    MUSHER, DM
    [J]. CLINICAL INFECTIOUS DISEASES, 1992, 14 (04) : 801 - 809
  • [23] CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE STREPTOCOCCUS-PNEUMONIAE GENE ENCODING PNEUMOLYSIN
    PATON, JC
    BERRY, AM
    LOCK, RA
    HANSMAN, D
    MANNING, PA
    [J]. INFECTION AND IMMUNITY, 1986, 54 (01) : 50 - 55
  • [24] THE IS4 FAMILY OF INSERTION SEQUENCES - EVIDENCE FOR A CONSERVED TRANSPOSASE MOTIF
    REZSOHAZY, R
    HALLET, B
    DELCOUR, J
    MAHILLON, J
    [J]. MOLECULAR MICROBIOLOGY, 1993, 9 (06) : 1283 - 1295
  • [25] IS861, A GROUP-B STREPTOCOCCAL INSERTION-SEQUENCE RELATED TO IS150 AND IS3 OF ESCHERICHIA-COLI
    RUBENS, CE
    HEGGEN, LM
    KUYPERS, JM
    [J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (10) : 5531 - 5535
  • [26] NUCLEOTIDE-SEQUENCE OF THE DEXTRAN GLUCOSIDASE (DEXB)-GENE OF STREPTOCOCCUS-MUTANS
    RUSSELL, RRB
    FERRETTI, JJ
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 : 803 - 810
  • [27] DESTRUCTION OF LOW EFFICIENCY MARKERS IS A SLOW PROCESS OCCURRING AT A HETERODUPLEX STAGE OF TRANSFORMATION
    SHOEMAKER, NB
    GUILD, WR
    [J]. MOLECULAR AND GENERAL GENETICS, 1974, 128 (04): : 283 - 290
  • [28] INTERRUPTION OF CAPSULE PRODUCTION IN STREPTOCOCCUS-PNEUMONIAE SEROTYPE-3 BY INSERTION OF TRANSPOSON TN916
    WATSON, DA
    MUSHER, DM
    [J]. INFECTION AND IMMUNITY, 1990, 58 (09) : 3135 - 3138
  • [29] ISOLATION AND DETECTION OF UREASE GENES IN UREAPLASMA-UREALYTICUM
    WILLOUGHBY, JJ
    RUSSELL, WC
    THIRKELL, D
    BURDON, MG
    [J]. INFECTION AND IMMUNITY, 1991, 59 (07) : 2463 - 2469