3-DIMENSIONAL ARCHITECTURE OF HUMAN ALPHA-2-MACROGLOBULIN TRANSFORMED WITH METHYLAMINE

被引：42
作者：
BOISSET, N
PENCZEK, P
POCHON, F
FRANK, J
LAMY, J
机构：
[1] CNRS, URA 1334, F-37042 TOURS, FRANCE
[2] NEW YORK STATE DEPT HLTH, WADSWORTH CTR LABS & RES, ALBANY, NY 12201 USA
[3] INST CURIE, INSERM, U219, F-91405 ORSAY, FRANCE
[4] SUNY Albany, DEPT BIOMED SCI, ALBANY, NY 12222 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 232卷 / 02期
关键词：
ALPHA-2-MACROGLOBULIN; CRYOELECTRON MICROSCOPY; FROZEN HYDRATED SPECIMEN; 3-DIMENSIONAL RECONSTRUCTION; MOLECULAR ARCHITECTURE;
D O I：
10.1006/jmbi.1993.1408
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A frozen-hydrated sample embedded in vitreous ice of human α2-macroglobulin transformed by methylamine was imaged by cryoelectron microscopy and reconstructed in three dimensions. In the reconstruction, the cage-like architecture of this protease inhibitor is fully revealed with a clear visualization of two lozenge-shaped lateral walls connected by thin bridges. The shape and dimensions of the internal cavity normally containing the trapped protease(s) is described. The possible locations of the thiol ester sites and intersubunit connections are also discussed.
引用
收藏
页码：522 / 529
页数：8
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