BIOCHEMICAL-EVIDENCE FOR THE PRESENCE OF ARGININE DECARBOXYLASE ACTIVITY IN TRYPANOSOMA-CRUZI

被引：44

作者：

MAJUMDER, S ^{[1
]}

WIRTH, JJ ^{[1
]}

BITONTI, AJ ^{[1
]}

MCCANN, PP ^{[1
]}

KIERSZENBAUM, F ^{[1
]}

机构：

[1] MARION MERRELL DOW RES INST,CINCINNATI,OH 45215

来源：

JOURNAL OF PARASITOLOGY | 1992年 / 78卷 / 02期

关键词：

D O I：

10.2307/3283493

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Trypanosoma cruzi was found to release 14CO2 from radiolabeled arginine, and this effect was inhibited by either DL-alpha-difluoromethylarginine or monofluoromethylagmatine, both specific inhibitors of arginine decarboxylase (ADC). Furthermore, agmatine, which can be derived metabolically only by ADC-mediated arginine decarboxylation, was produced when T. cruzi was incubated with radiolabeled arginine, and agmatine production was inhibited in the presence of DL-alpha-difluoromethylarginine. These results constitute direct biochemical evidence for the presence in T. cruzi of ADC, an enzyme that does not occur in mammalian cells.

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页码：371 / 374

页数：4

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