MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY OF OLIGODEOXYTHYMIDYLIC ACIDS

被引：53

作者：

HUTHFEHRE, T ^{[1
]}

GOSINE, JN ^{[1
]}

WU, KJ ^{[1
]}

BECKER, CH ^{[1
]}

机构：

[1] SRI INT,MOLEC PHYS LAB,333 RAVENSWOOD AVE,MENLO PK,CA 94025

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 03期

关键词：

D O I：

10.1002/rcm.1290060312

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ferulic acid, sinapinic acid and 2,5-dihydroxybenzoic acid (DHBA) have been tested as matrix materials for matrix-assisted laser desorption of the pure oligonucleotide pd(T)12 and a mixture of oligonucleotides pd(T)12 through pd(T)18 using pulsed 337 nm radiation combined with reflecting time-of-flight mass spectrometry. The three matrix materials are compared with respect to obtainable mass resolution, degree of fragmentation, and adduct formation for these oligonucleotides. DHBA was found to produce the least fragmentation and adduct formation, as well as the highest mass resolution.

引用

页码：209 / 213

页数：5

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