MAPPING OF RNA BY A MODIFICATION OF THE BERK-SHARP PROCEDURE - 5' TERMINI OF 15-S BETA-GLOBIN MESSENGER-RNA PRECURSOR AND MATURE 10-S BETA-GLOBIN MESSENGER-RNA HAVE IDENTICAL MAP COORDINATES

We have used a modification of the Berk-Sharp technique to determine that the 5′ termini of the mouse 15 S β-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5′ (or 3′) terminally labeled probes; it allows the detection of the precursor in the presence of excess nature mRNA. © 1979 Information Retrieval Limited.