MAPPING OF RNA BY A MODIFICATION OF THE BERK-SHARP PROCEDURE - 5' TERMINI OF 15-S BETA-GLOBIN MESSENGER-RNA PRECURSOR AND MATURE 10-S BETA-GLOBIN MESSENGER-RNA HAVE IDENTICAL MAP COORDINATES

被引：1186

作者：

WEAVER, RF ^{[1
]}

WEISSMANN, C ^{[1
]}

机构：

[1] UNIV ZURICH,INST MOLEK BIOL 1,CH-8093 ZURICH,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 05期

关键词：

D O I：

10.1093/nar/7.5.1175

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a modification of the Berk-Sharp technique to determine that the 5′ termini of the mouse 15 S β-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5′ (or 3′) terminally labeled probes; it allows the detection of the precursor in the presence of excess nature mRNA. © 1979 Information Retrieval Limited.

引用

页码：1175 / 1193

页数：19

共 34 条

[21] ANALYSIS OF RESTRICTION FRAGMENTS OF T7 DNA AND DETERMINATION OF MOLECULAR-WEIGHTS BY ELECTROPHORESIS IN NEUTRAL AND ALKALINE GELS [J].

MCDONELL, MW ;

SIMON, MN ;

STUDIER, FW .

JOURNAL OF MOLECULAR BIOLOGY, 1977, 110 (01) :119-146

[22] PRECURSOR OF GLOBIN MESSENGER-RNA - APPENDIX - SOLUTION HYBRIDIZATION ASSAY TO DETECT RADIOACTIVE GLOBIN MESSENGER-RNA NUCLEOTIDE-SEQUENCES [J].

ROSS, J .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 106 (02) :403-420

[23] PURIFICATION AND STRUCTURAL-PROPERTIES OF PRECURSORS OF GLOBIN MESSENGER-RNAS [J].

ROSS, J .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 119 (01) :21-35

[24] PRECURSORS OF ALPHA AND BETA GLOBIN MESSENGER-RNAS [J].

ROSS, J ;

KNECHT, DA .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 119 (01) :1-20

[25]

SCHAMBOCK A, 1979, EXPERIENTIA, V35, P978

[26] SEQUENCE ORGANIZATION OF BETA-GLOBIN MESSENGER-RNA PRECURSOR [J].

SMITH, K ;

LINGREL, JB .

NUCLEIC ACIDS RESEARCH, 1978, 5 (09) :3295-3301

[27] CHARACTERIZATION OF GLOBIN MESSENGER-RNA SEQUENCES IN NUCLEUS OF DUCK IMMATURE RED BLOOD-CELLS [J].

STRAIR, RK ;

SKOULTCHI, AI ;

SHAFRITZ, DA .

CELL, 1977, 12 (01) :133-141

[28] CLONING SPECIFIC SEGMENTS OF MAMMALIAN GENOME - BACTERIOPHAGE-LAMBDA CONTAINING MOUSE GLOBIN AND SURROUNDING GENE SEQUENCES [J].

TILGHMAN, SM ;

TIEMEIER, DC ;

POLSKY, F ;

EDGELL, MH ;

SEIDMAN, JG ;

LEDER, A ;

ENQUIST, LW ;

NORMAN, B ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4406-4410

[29] INTERVENING SEQUENCE OF A MOUSE BETA-GLOBIN GENE IS TRANSCRIBED WITHIN 15S BETA-GLOBIN MESSENGER-RNA PRECURSOR [J].

TILGHMAN, SM ;

CURTIS, PJ ;

TIEMEIER, DC ;

LEDER, P ;

WEISSMANN, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (03) :1309-1313

[30] STRUCTURAL-ANALYSIS OF THE FIBROIN GENE AT THE 5' END AND ITS SURROUNDING REGIONS [J].

TSUJIMOTO, Y ;

SUZUKI, Y .

CELL, 1979, 16 (02) :425-436

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