MAPPING OF RNA BY A MODIFICATION OF THE BERK-SHARP PROCEDURE - 5' TERMINI OF 15-S BETA-GLOBIN MESSENGER-RNA PRECURSOR AND MATURE 10-S BETA-GLOBIN MESSENGER-RNA HAVE IDENTICAL MAP COORDINATES

被引：1186

作者：

WEAVER, RF ^{[1
]}

WEISSMANN, C ^{[1
]}

机构：

[1] UNIV ZURICH,INST MOLEK BIOL 1,CH-8093 ZURICH,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 05期

关键词：

D O I：

10.1093/nar/7.5.1175

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a modification of the Berk-Sharp technique to determine that the 5′ termini of the mouse 15 S β-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5′ (or 3′) terminally labeled probes; it allows the detection of the precursor in the presence of excess nature mRNA. © 1979 Information Retrieval Limited.

引用

页码：1175 / 1193

页数：19

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