CA2+ BINDING AND EXTRACTION OF 4-HOUR-AGED RAT MITOCHONDRIA

被引：10

作者：

OTA, A ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL CHEM,BALTIMORE,MD 21205

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1979年 / 10卷 / 12期

关键词：

D O I：

10.1016/0020-711X(79)90085-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. High affinity Ca2+ binding was considerably inhibited by antimycin A + rotenone in 4-hr-aged rat liver mitochondria. 2. 2. Two kinds of high-affinity binding sites, energy independent and dependent or inhibitor-sensitive and a small amount of Ca2+ transport, are supposed to exist. 3. 3. 28, 41, 2 and 5% of the loaded Ca2+ was extracted with the stepwise extraction with 0.25 M sucrose, distilled water, 0.2 M KCl, and chloroform-methanol (2:1), respectively and 24% of the Ca2+ remained in the mitochondria. © 1979.

引用

页码：1033 / 1037

页数：5

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