INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE ACTIVITY IN FROG SKELETAL-MUSCLE

被引：12
|
作者：
CARRASCO, MA [1 ]
FIGUEROA, S [1 ]
机构：
[1] CTR ESTUDIOS CIENT SANTIAGO,SANTIAGO 9,CHILE
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1995年 / 110卷 / 04期
关键词：
CALCIUM ION; CALMODULIN; CAUDIVERBERA CAUDIVERBERA; FROG SKELETAL MUSCLE; IP3; 3-KINASE; 5-PHOSPHATASE;
D O I：
10.1016/0305-0491(94)00197-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Frog skeletal muscle contains a kinase activity that phosphorylates inositol 1,4,5-trisphosphate to inositol 1,3,4,5-tetrakisphosphate. The inositol 1,4,5-trisphosphate 3-kinase activity was mainly recovered in the soluble fraction, where it presented a marked dependency on free calcium concentration in the physiological range in the presence of endogenous calmodulin. At pCa 5, where the activity was highest, the soluble 3-kinase activity displayed a K-m for inositol 1,4,5-trisphosphate of 1.6 mu M and a V-max value of 25.1 pmol mg(-1) min(-1). The removal rates of inositol 1,4,5-trisphosphate by 3-kinase and 5-phosphatase activities of the total homogenate under physiological ionic conditions were very similar, suggesting that both routes are equally important in metabolizing inositol 1,4,5-trisphosphate in frog skeletal muscle.
引用
收藏
页码：747 / 753
页数：7
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