COMPARISON OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND BIOASSAY OF AMPHOTERICIN-B IN SERUM

被引：0
作者：
HULSEWEDE, JW [1 ]
DERMOUMI, H [1 ]
机构：
[1] UNIV ESSEN GESAMTHSCH KLINIKUM,INST MED MICROBIOL,D-45122 ESSEN,GERMANY
来源：
MYCOSES | 1994年 / 37卷 / 1-2期
关键词：
ANTIFUNGALS; AMPHOTERICIN B; SERUM CONCENTRATION; HPLC; BIOASSAY;
D O I：
暂无
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
For the determination of amphotericin B in serum a high-performance liquid chromatography (HPLC) method using simple protein precipitation and an isocratic mobile phase as well as a plate diffusion bioassay are described. Using 5-fluorocytosine-resistant strain of Candida albicans and peptone in medium as an antagonist of 5-fluorocytosine allows simple measurement of amphotericin B both in the absence and in the presence of 5-fluorocytosine. The correlation coefficient (r) between bioassay and HPLC runs is 0.88. Generally, the biological determination gives slightly higher values of amphotericin B than the HPLC method. Both methods are useful for amphotericin B drug monitoring.
引用
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页码：17 / 21
页数：5
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