A NEW DOUBLE LABELING TECHNIQUE OF THE SEROTONERGIC NEURONS IN WHOLEMOUNT CNS PREPARATIONS OF THE SNAIL HELIX-POMATIA

被引:0
|
作者
HERNADI, L
机构
来源
ACTA BIOLOGICA HUNGARICA | 1991年 / 42卷 / 04期
关键词
SEROTONERGIC NEURONS; DOUBLE LABELING; 5,6-DHT; COBALT; NICKEL HELIX-POMATIA;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
5,6-dihydroxytryptamine-induced pigmentation of serotonergic neurons was combined with retrograde cobalt- or nickel-lysine labelling in order to identify serotonergic cell bodies among cobalt- or nickel-labelled ones. Cobalt- or nickel-labelled serotonergic cell bodies could easily be distinguished from labelled, nonserotonergic neurons. The nonserotonergic cobalt-labelled neurons have only orange, whereas the nickel-labelled neurons have only blue colorization in their somata, while the labelled serotonergic neurons have dark pigment granules in their somata besides the blue or orange colorization. By this double labelling technique the central serotonergic neurons which send processes to different body regions through CNS nerves, can be identified in wholemount preparations.
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页码:417 / 423
页数:7
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