AMPLIFICATION OF GENOMIC SEQUENCES FLANKING TRANSPOSABLE ELEMENTS IN HOST AND HETEROLOGOUS PLANTS - A TOOL FOR TRANSPOSON TAGGING AND GENOME CHARACTERIZATION

被引：44
作者：
EARP, DJ
LOWE, B
BAKER, B
机构：
[1] UNIV CALIF BERKELEY,USDA,CTR PLANT GENE EXPRESS,800 BUCHANAN ST,ALBANY,CA 94710
[2] UNIV CALIF BERKELEY,DEPT PLANT PATHOL,BERKELEY,CA 94720
来源：
NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 11期
关键词：
D O I：
10.1093/nar/18.11.3271
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated Into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was established in a model system, a transgenic tobacco plant carrying a single Ac element, and successfully applied to the cloning of a specific Spm element from a maize line carrying multiple Spm hybridizing sequences. The described utilization of methylation sensitive restriction enzymes (including those with degenerate recognition sequences) in the generation of templates for amplification will simplify the cloning and mapping of genomic sequences adjacent to transposable elements. © 1990 Oxford University Press.
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页码：3271 / 3279
页数：9
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