ISOLATION OF AN ACTIVATOR FOR PROSTAGLANDIN HYDROPEROXIDASE FROM BOVINE VESICULAR GLAND CYTOSOL AND ITS IDENTIFICATION AS URIC-ACID

被引：45
作者：
OGINO, N
YAMAMOTO, S
HAYAISHI, O
TOKUYAMA, T
机构：
[1] KYOTO UNIV,FAC MED,DEPT MED CHEM,SAKYO KU,KYOTO 606,JAPAN
[2] OSAKA CITY UNIV,FAC SCI,DEPT CHEM,OSAKA 558,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 01期
关键词：
D O I：
10.1016/0006-291X(79)91664-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzymatic conversion of prostaglandin G1 to H1 was stimulated by an activator present in the cytosol of bovine vesicular gland. The activator was purified by Sephadex G-25 gel filtration and Dowex 1 column chromatography. The purified activator was identified to be uric acid by thin layer chromatography, ultraviolet and infrared absorption spectroscopy and combined gas chromatography-mass spectroscopy. Among various purine compounds tested, only uric acid and 2,8-dihydroxyadenine were active. © 1979.
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页码：184 / 191
页数：8
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