Implementation of a robotized real-time PCR setup for the use of the Quantifiler (TM) Human DNA Quantification Kit

被引：6

作者：

Schwenzer, Ralph ^{[1
]}

Schoeneberg, Annette ^{[1
]}

Pflug, Werner ^{[1
]}

机构：

[1] Kriminaltech Inst, Landeskriminalamt Baden Wurttemberg, Taubenheimstr 85, D-70372 Stuttgart, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2008年 / 1卷 / 01期

关键词：

Automation; DNA quantification; Forensic casework samples; Real-time PCR; Quantifiler (TM) Human DNA Quantification Kit;

D O I：

10.1016/j.fsigss.2007.10.151

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human DNA quantification occupies a central role within the DNA analytic process of forensic casework samples as DNA quantification results have an important impact on the quality of the short tandem repeat data. Manual processing for the setup of quantification reactions can be time consuming and labor intensive. Therefore automation of quantitative real-time PCR setup was an important component of our DNA-analysis automation concept. Here we show the implementation of a robotized setup for the Quantifiler (TM) Human DNA Quantification Kit. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：68 / 70

页数：3