CHARACTERIZATION OF A HUMAN CHROMOSOME-4 FLOW-SORTED COSMID LIBRARY

被引：19
作者：
RIESS, O [1 ]
SIEDLACZCK, I [1 ]
KREDTKE, S [1 ]
MELMER, G [1 ]
EPPLEN, JT [1 ]
DEAVEN, LL [1 ]
机构：
[1] LOS ALAMOS NATL LAB, LOS ALAMOS, NM 87545 USA
来源：
CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 04期
关键词：
D O I：
10.1159/000133638
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 2 5,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.
引用
收藏
页码：238 / 242
页数：5
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