OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF BACERIAL CHITINASE ISOLATED FROM MARINE CRUSTACEAN SHELLS

被引：0
|
作者：
Venkatraman, Anuradha [1 ]
Yacoob, Syed Ali Mohamed [2 ]
Nagarajan, Yogananth [2 ]
Mohamed, P. Kalitha Parveen Peer [2 ]
机构：
[1] Mohamed Sathak Coll Arts & Sci, PG & Res Dept Biochem, Chennai 600119, India
[2] Mohamed Sathak Coll Arts & Sci, PG & Res Dept Biotechnol, Chennai 600119, India
来源：
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES | 2014年 / 3卷 / 04期
关键词：
Crustacean shells; bacterial chitinase; chitin; optimization;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Six bacterial isolates identified from marine crustacean shell wastes' were studied under different growth parameters to optimize the production of chitinase. The enzyme activity was determined with different growth media and optimized under different conditions of pH, Temperature and substrate concentration. The enzyme activity was found to be maximum in nutrient broth with 0.3% colloidal chitin. The optimum pH and temperature were found to vary between the isolates. Under optimized condition, the chitinase activity was doubled than in the normal condition.
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页码：319 / 321
页数：3
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