Histomorphology, Sperm Quality and Hormonal Profile in Adult Male Sprague-Dawley Rats following administration of aqueous crude Extract of Solanum nigrum by gastric gavage

Objective: The present study focused on histomorphology, sperm quality, hormonal profile and hematological parameters in adult male Sprague-Dawley rats following the administration of aqueous crude extract of Solanum nigrum by gastric gavage. Methods: Fourty healthy male adult (12-14 weeks old) Sprague-Dawley rats weighing 200-220g were randomly divided into four groups (A, B, C and D) of ten (n=10) rats each. Group A which served as control were given distilled water 2ml/kg b.wt each, daily for 28 days. Group B, C and D rats were administered 100, 300 and 500mg/kg b.wt each daily respectively for 28 days. The extract was saved with LD50 > 5000mg/Kg. Sperm counts, percentage motility, morphology and percentage live sperm, hormonal profile and hematological parameter were quantified; testis, epididymal and general body weights were measured using a weighing scale. The extract was administered once daily for six days within a week via oral gavage. After the last administration, all rats were sacrificed by cervical dislocation, the testis were harvested and fixed in Bouin's fluid for histology processing. Results: Our results revealed an increase in sperm counts, percentage of motility, morphology and percentage of live sperm, blood level of follicle stimulating hormone, Luteinizing hormone and testosterone, hematological parameters, testis, epididymal and general body weights across the groups in a dose-depentant manner. The testis histoarchtecture showed normal cellular composition in their germinal epithelium, with sperm cells in the lumen and a normal interstitium. Conclusion: This experiment revealed that aqueous extract of Solanum nigrum bears profertility properties which may be beneficial to those who consume it.