DIFFERENTIAL PP60C-SRC ACTIVITY IN WELL AND POORLY DIFFERENTIATED HUMAN COLON CARCINOMAS AND CELL-LINES

被引：61

作者：

WEBER, TK ^{[1
]}

STEELE, G ^{[1
]}

SUMMERHAYES, IC ^{[1
]}

机构：

[1] NEW ENGLAND DEACONESS HOSP,DEPT SURG,CANC BIOL LAB,50 BINNEY ST,BOSTON,MA 02215

来源：

JOURNAL OF CLINICAL INVESTIGATION | 1992年 / 90卷 / 03期

关键词：

PP60C-SRC; COLON CARCINOMA; DIFFERENTIATION;

D O I：

10.1172/JCI115956

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The results presented in this report demonstrate increased pp60c-src kinase activity associated with moderate to well differentiated colon tumors, corroborating previous observations by other groups. Extension of this analysis to include a small number of poorly differentiated colon carcinomas revealed src kinase activity comparable to that observed in normal colonic mucosa, considerably less than that observed in moderate / well differentiated lesions. Correlations of src kinase activity with differentiation was confirmed within a panel of colon cell lines where increased activity, associated with moderate / well differentiated lines, was accompanied by increased expression of pp60c-src protein. Use of an antiphosphotyrosine antibody in immunoprecipitation revealed the presence of novel phosphotyrosyl cellular substrates in human colon cell lines displaying elevated pp60c-src kinase activity. These observations suggest a role for the src protooncogene in colonic differentiation pathways.

引用

页码：815 / 821

页数：7

共 37 条

[1] ALTERED TYROSINE-527 PHOSPHORYLATION AND MITOTIC ACTIVATION OF P60C-SRC [J].

BAGRODIA, S ;

CHACKALAPARAMPIL, I ;

KMIECIK, TE ;

SHALLOWAY, D .

NATURE, 1991, 349 (6305) :172-175

[2] CHROMOSOME-17 DELETIONS AND P53 GENE-MUTATIONS IN COLORECTAL CARCINOMAS [J].

BAKER, SJ ;

FEARON, ER ;

NIGRO, JM ;

HAMILTON, SR ;

PREISINGER, AC ;

JESSUP, JM ;

VANTUINEN, P ;

LEDBETTER, DH ;

BARKER, DF ;

NAKAMURA, Y ;

WHITE, R ;

VOGELSTEIN, B .

SCIENCE, 1989, 244 (4901) :217-221

[3] EARLY ACTIVATION OF ENDOGENOUS PP60SRC KINASE-ACTIVITY DURING NEURONAL DIFFERENTIATION OF CULTURED HUMAN NEUROBLASTOMA-CELLS [J].

BJELFMAN, C ;

MEYERSON, G ;

CARTWRIGHT, CA ;

MELLSTROM, K ;

HAMMERLING, U ;

PAHLMAN, S .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (01) :361-370

[4] INCREASED PP60C-SRC TYROSYL KINASE-ACTIVITY IN HUMAN NEUROBLASTOMAS IS ASSOCIATED WITH AMINO-TERMINAL TYROSINE PHOSPHORYLATION OF THE SRC GENE-PRODUCT [J].

BOLEN, JB ;

ROSEN, N ;

ISRAEL, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7275-7279

[5] ACTIVATION OF PP60C-SRC PROTEIN-KINASE ACTIVITY IN HUMAN-COLON CARCINOMA [J].

BOLEN, JB ;

VEILLETTE, A ;

SCHWARTZ, AM ;

DESEAU, V ;

ROSEN, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) :2251-2255

[6] PREVALENCE OF RAS GENE-MUTATIONS IN HUMAN COLORECTAL CANCERS [J].

BOS, JL ;

FEARON, ER ;

HAMILTON, SR ;

VERLAANDEVRIES, M ;

VANBOOM, JH ;

VANDEREB, AJ ;

VOGELSTEIN, B .

NATURE, 1987, 327 (6120) :293-297

[7] CHARACTERIZATION OF THE ALTERED FORM OF THE C-SRC GENE-PRODUCT IN NEURONAL CELLS [J].

BRUGGE, J ;

COTTON, P ;

LUSTIG, A ;

YONEMOTO, W ;

LIPSICH, L ;

COUSSENS, P ;

BARRETT, JN ;

NONNER, D ;

KEANE, RW .

GENES & DEVELOPMENT, 1987, 1 (03) :287-296

[8] NEURONS EXPRESS HIGH-LEVELS OF A STRUCTURALLY MODIFIED, ACTIVATED FORM OF PP60C-SRC [J].

BRUGGE, JS ;

COTTON, PC ;

QUERAL, AE ;

BARRETT, JN ;

NONNER, D ;

KEANE, RW .

NATURE, 1985, 316 (6028) :554-557

[9] PP60C-SRC EXPRESSION IN THE DEVELOPING RAT-BRAIN [J].

CARTWRIGHT, CA ;

SIMANTOV, R ;

COWAN, WM ;

HUNTER, T ;

ECKHART, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) :3348-3352

[10] CELL-TRANSFORMATION BY PP60C-SRC MUTATED IN THE CARBOXY-TERMINAL REGULATORY DOMAIN [J].

CARTWRIGHT, CA ;

ECKHART, W ;

SIMON, S ;

KAPLAN, PL .

CELL, 1987, 49 (01) :83-91

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